Improvement of Soybean Agrobacterium-Mediated Transformation Efficiency by Adding Glutamine and Asparagine into the Culture Media

文献类型： 外文期刊

第一作者： Chen, Li

作者： Chen, Li;Cai, Yupeng;Liu, Xiujie;Yao, Weiwei;Guo, Chen;Hou, Wensheng;Chen, Li;Cai, Yupeng;Liu, Xiujie;Yao, Weiwei;Guo, Chen;Sun, Shi;Wu, Cunxiang;Jiang, Bingjun;Han, Tianfu;Hou, Wensheng

作者机构：

关键词： soybean; Agrobacterium-mediated transformation; l-asparagine; l-glutamine; transformation frequency

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2018 年 19 卷 10 期

页码：

收录情况： SCI

摘要： As a genetically modified crop, transgenic soybean occupies the largest global scale with its food, nutritional, industrial, and pharmaceutical uses.Efficient transformation is a key factor for the improvement of genetically modified soybean. At present, the Agrobacterium-mediated method is primarily used for soybean transformation, but the efficiency of this method is still relatively low (below 5%) compared with rice (above 90%). In this study, we examined the influence of l-glutamine and/or l-asparagine on Agrobacterium-mediated transformation in soybean and explored the probable role in the process of Agrobacterium-mediated transformation. The results showed that when the amino acids l-glutamine and l-asparagine were added separately or together to the culture medium, the shoot induction frequency, elongation rate, and transformation frequency were improved. The combined effects of l-glutamine and l-asparagine were better than those of l-glutamine and l-asparagine alone. The 50 mg/L l-glutamine and 50 mg/L l-asparagine together can enhance the transformation frequency of soybean by attenuating the expression level of GmPRs (GmPR1, GmPR4, GmPR5, and GmPR10) and suppression of the plant defense response. The transgene was successfully transmitted to the T1 generation. This study will be useful in genetic engineering of soybean.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序