Suppression of nbe-miR166h-p5 attenuates leaf yellowing symptoms of potato virus X on Nicotiana benthamiana and reduces virus accumulation

文献类型： 外文期刊

第一作者： Wang, Shu

作者： Wang, Shu;Cui, Weijun;Wu, Xinyang;Chen, Jianping;Wang, Shu;Cui, Weijun;Wu, Xinyang;Yuan, Quan;Zhao, Jinping;Zheng, Hongying;Lu, Yuwen;Peng, Jiejun;Lin, Lin;Chen, Jianping;Yan, Fei;Wang, Shu;Cui, Weijun;Wu, Xinyang;Yuan, Quan;Zhao, Jinping;Zheng, Hongying;Lu, Yuwen;Peng, Jiejun;Lin, Lin;Chen, Jianping;Yan, Fei;Yuan, Quan;Chen, Jianping;Yan, Fei

作者机构：

关键词： chlorophyll; leaf yellowing; miRNA; nbe-miR166h-p5; Potato virus X

期刊名称：MOLECULAR PLANT PATHOLOGY （ 影响因子：5.663； 五年影响因子：5.626 ）

ISSN： 1464-6722

年卷期： 2018 年 19 卷 11 期

页码：

收录情况： SCI

摘要： MicroRNAs (miRNAs) play essential roles in plant development. There is increasing evidence that changed expression of miRNAs in virus-infected plants contributes to the development of viral symptoms. Here, we analysed the altered expression of miRNAs of Nicotiana benthamiana in response to Potato virus X (PVX) by Illumina Solexa sequencing. One of the 21 miRNAs significantly affected, nbe-miR166h-p5, was closely associated with viral symptoms. Using the Tobacco rattle virus-based miRNA suppression (VbMS) system, we found that the suppression of nbe-miR166h-p5 in plants caused leaves to turn dark green with increased chlorophyll. When PVX was inoculated on nbe-miR166h-p5-suppressed plants, the leaf yellowing symptom of PVX was largely attenuated with less reduction in chlorophyll content, and the accumulation of PVX was decreased. nbe-miR166h-p5 was also up-regulated in plants infected by Turnip mosaic virus (TuMV), and its suppression attenuated the leaf yellowing symptom of TuMV and decreased viral accumulation. Three potential targets of nbe-miR166h-p5 were identified. The results indicate the association of nbe-miR166h-p5 with symptoms of PVX and also with those of TuMV, providing useful information on the relationship between miRNA and viral infection.

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