Proteomic Analysis of the Function of a Novel Cold-Regulated Multispanning Transmembrane Protein COR413-PM1 in Arabidopsis

文献类型： 外文期刊

第一作者： Su, Chen

作者： Su, Chen;Yang, Rui;Xi, Yajun;Su, Chen;Chen, Kai;Ding, Qingqian;Mou, Yongying;Yang, Rui;Zhao, Mengjie;Ma, Bo;Xu, Zhaoshi;Ma, Youzhi;Pan, Yinghong;Chen, Ming;Xi, Yajun;Su, Chen;Chen, Kai;Ding, Qingqian;Yang, Rui;Xu, Zhaoshi;Ma, Youzhi;Chen, Ming;Xi, Yajun;Chen, Kai

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关键词： freezing stress; COR413-PM1; gene function; proteomic analysis; Arabidopsis

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2018 年 19 卷 9 期

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收录情况： SCI

摘要： The plasma membrane is the first subcellular organ that senses low temperature, and it includes some spanning transmembrane proteins that play important roles in cold regulation. COR413-PM1 is a novel multispanning transmembrane cold-regulated protein; however, the related functions are not clear in Arabidopsis. We found the tolerance to freezing stress of cor413-pm1 was lower than wild-type (WT). A proteomics method was used to analyze the differentially abundant proteins (DAPs) between cor413-pm1 and WT. A total of 4143 protein groups were identified and 3139 were accurately quantitated. The DAPs associated with COR413-PM1 and freezing treatment were mainly involved in the metabolism of fatty acids, sugars, and purine. Quantitative real-time PCR (qRT-PCR) confirmed the proteomic analysis results of four proteins: fatty acid biosynthesis 1 (FAB1) is involved in fatty acid metabolism and might affect the plasma membrane structure; fructokinase 3 (FRK3) and sucrose phosphate synthase A1 (SPSA1) play roles in sugar metabolism and may influence the ability of osmotic adjustment under freezing stress; and GLN phosphoribosyl pyrophosphate amidotransferase 2 (ASE2) affects freezing tolerance through purine metabolism pathways. In short, our results demonstrate that the multispanning transmembrane protein COR413-PM1 regulates plant tolerance to freezing stress by affecting the metabolism of fatty acids, sugars, and purine in Arabidopsis.

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