Recombinant Escherichia coli BL21-pET28a-egfp Cultivated with Nanomaterials in a Modified Microchannel for Biofilm Formation

文献类型： 外文期刊

第一作者： Zhu, Chang-Tong

作者： Zhu, Chang-Tong;Mei, Yi-Yuan;Zhu, Lin-Lin;Xu, Yan;Sheng, Sheng;Wang, Jun;Zhu, Chang-Tong;Mei, Yi-Yuan;Zhu, Lin-Lin;Xu, Yan;Sheng, Sheng;Wang, Jun;Xu, Yan;Sheng, Sheng;Wang, Jun

作者机构：

关键词： biofilm; microreactor; surface modification; nanomaterials; recombinant Escherichia coli

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2018 年 19 卷 9 期

页码：

收录情况： SCI

摘要： The application of whole cells as catalytic biofilms in microchannels has attracted increasing scientific interest. However, the excessive biomass formation and structure of biofilms in a reactor limits their use. A microchannel reactor with surface modification was used to colonize recombinant Escherichia coil BL21-pET28a-egfp rapidly and accelerated growth of biofilms in the microchannel. The segmented flow system of 'air/culture medium containing nanomaterials' was firstly used to modulate the biofilms formation of recombinant E. coli; the inhibitory effects of nanomaterials on biofilm formation were investigated. The results indicated that the segmental flow mode has a significant impact on the structure and development of biofilms. Using the channels modified by silane reagent, the culture time of biofilms (30 h) was reduced by 6 h compared to unmodified channels. With the addition of graphene sheets (10 mg/L) in Luria-Bertani (LB) medium, the graphene sheets possessed a minimum inhibition rate of 3.23% against recombinant E. coli. The biofilms cultivated by the LB medium with added graphene sheets were stably formed in 20 h; the formation time was 33.33% shorter than that by LB medium without graphene. The developed method provides an efficient and simple approach for rapid preparation of catalytic biofilms in microchannel reactors.

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