Identification and comparative analysis of the miRNA expression profiles from four tissues of Micropterus salmoides using deep sequencing

文献类型： 外文期刊

第一作者： Gong, Wangbao

作者： Gong, Wangbao;Xie, Jun;Wang, Guangjun;Yu, Deguang;Zhang, Kai;Li, Zhifei;Yu, Ermeng;Tian, Jingjing;Zhu, Yun;Huang, Yong;Xie, Jun;Wang, Guangjun;Yu, Deguang;Zhang, Kai;Li, Zhifei;Yu, Ermeng;Tian, Jingjing;Zhu, Yun

作者机构：

关键词： Micropterus salmoides; miRNA; Deep sequencing; Expression

期刊名称：GENOMICS （ 影响因子：5.736； 五年影响因子：4.939 ）

ISSN： 0888-7543

年卷期： 2018 年 110 卷 6 期

页码：

收录情况： SCI

摘要： In the present study, four small RNA libraries were constructed from an M. salmoides population and sequenced using deep sequencing technology. A total of 9,888,822; 8,519,365; 20,566,198; and 15,762,254 raw reads representing 666,097; 755,711; 978,923; and 840,175 unique sequences were obtained from the spleen, liver, kidney, and muscle libraries, respectively. As a result, 509 known miRNAs belonging to 143 families and 1157 novel miRNAs were identified. The miRNAs displayed diverse expression levels among the four libraries, among which most of the known miRNAs were expressed at higher levels than the novel miRNAs. Furthermore, stemloop qRT-PCR was applied to validate and profile the expression of the differentially expressed miRNAs in the four different tissues, which revealed that some miRNAs showed tissue specific expression. The identification of miRNAs in M. salmoides will provide new information and enhance our understanding of the functions of miRNAs in regulating biological processes.

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