Synthesis and Application of Water-Soluble Oxazine Dyes for Detection of PHAs-Producing Bacteria

文献类型： 外文期刊

第一作者： Liu, Qing-Hao

作者： Liu, Qing-Hao;Lu, Hong-Yu;Guo, Yan-Nan;Wang, Hai-Bin;Hu, Zhi-Yong;Dong-Liang;Guo, Jin-Chun;Liu, Hong-Yan;Chen, Li-Gong

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关键词： Oxazine dyes; Carboxyl groups; Good solubility; Staining method; PHAs-producing bacteria

期刊名称：JOURNAL OF FLUORESCENCE （ 影响因子：2.217； 五年影响因子：1.906 ）

ISSN： 1053-0509

年卷期： 2018 年 28 卷 6 期

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收录情况： SCI

摘要： Derivatives of oxazine dyes were synthesized on mulitigram scales via efficient synthetic strategies. One practical route was selected to prepare compounds 6, 9 and 10, especially water-soluble compound 6 was obtained in better yield than reported, and compound 10 was insoluble in aqueous media in absence of phenolic-OH. Compounds 3 and 9 were found to be clear pH-dependent between pH=4.0 and 10.0, and could be used as acid-base indicators to measure intracellular pH. Compounds 6, 9, 10 all have carboxylic acid functionalities, which could be activated and used to conjugate the dyes to biomolecules. In addition, compounds 6 and 9 with good solubility in aqueous media were used to develop a simple, quick, safe, highly sensitive staining method to detect PHAs-producing bacteria on heat-fixed smears, which was confirmed by fluorescence images of PHAs granules of bacteria.

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