Multilineage potential research of Beijing duck amniotic mesenchymal stem cells

文献类型： 外文期刊

第一作者： Ma, Caiyun

作者： Ma, Caiyun;Ji, Hongda;Wang, Hongliang;Wang, Xishuai;Guan, Weijun;Wang, Kunfu;Guo, Liangcai;Wang, Zhiyong;Ren, Han

作者机构：

关键词： Beijing duck; Amniotic mesenchymal stem cells; Multiply differentiation; Biological characteristics

期刊名称：CELL AND TISSUE BANKING （ 影响因子：1.522； 五年影响因子：1.968 ）

ISSN： 1389-9333

年卷期： 2018 年 19 卷 4 期

页码：

收录情况： SCI

摘要： Amnion, which is usually discarded as medical waste, is considered as abundant sources for mesenchymal stem cells. In human and veterinary medicine, the multipotency of mesenchymal stem cells derived from amnion (AMSCs) together with their plasticity, self-renewal, low immunogenicity and nontumorigenicity characteristics make AMSCs a promising candidate cell for cell-based therapies and tissue engineering. However, up tillnow, the multipotential characteristics and therapeutic potential of AMSCs on preclinical studies remain uncertain. In this work, we successfully obtained AMSCs from Beijing duck embryos in vitro, and also attempted to detect their biological characteristics. The isolated AMSCs were phenotypically identified, the growth kinetics and karyotype were tested. Also, the cells were positive for MSCs-related markers (CD29, CD71, CD105, CD166, Vimentin and Fibronection), while the expression of CD34 and CD45 were undetectable. Additionally, AMSCs also expressed the pluripotent marker gene OCT4. Particularly, when appropriately induced, AMSCs could be induced to trans-differentiate into adipocytes, osteoblasts, chondrocytes and neurocytes in vitro. Together, these results demonstrated that the isolated AMSCs maintained their stemness and proliferation in vitro, which may be useful for future cell therapy in regenerative medicine.

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