Effect of ammonia-N on histology and expression of immunoglobulin M and component C3 in the spleen and head kidney of Pelteobagrus vachellii

文献类型： 外文期刊

第一作者： Shao, Ting

作者： Shao, Ting;Wang, Yong-ming;Yang, Qin;Bu, Ping;Shao, Ting;Gong, Quan

作者机构：

关键词： Ammonia; Pelteobagrus vachellii; Component C3; Immunoglobulin M; Lysozyme

期刊名称：AQUACULTURE REPORTS （ 影响因子：3.216； 五年影响因子：3.812 ）

ISSN： 2352-5134

年卷期： 2017 年 8 卷

页码：

收录情况： SCI

摘要： Ammonia-N is toxic to many aquatic animals and serves as a key stress factor in aquatic environments. The effects of ammonia-N stress on the immune response of darkbarbel catfish Pelteobagrus vachellii were investigated in this study. Changes in overall histology, and in the expression of complement C3 and immunoglobulin M (IgM) in spleen and head kidney, and lysozyme and C3 in serum, were measured in 1 and 5 mg/L ammonia-N. Hyperemia, melano-macrophage assembly and loose splenosis were evident in spleen tissue. Both lysozyme and component C3 were significantly reduced in serum (P < 0.05) under both stress treatments, although lysozyme was increased slightly at 24 h in the 1 mg/L treatment. C3 mRNA expression increased at 6 h in spleen and 6-12 h in head kidney then decreased rapidly (P < 0.05), although C3 mRNA recovered to control levels in spleen at 96 h after the 1 mg/L treatment (P > 0.05). IgM expression also increased significantly at 6-12 h in spleen and 6-24 h in head kidney after the 1 mg/L treatment (P < 0.05). A similar overall pattern were observed with 5 mg/L ammonia (P < 0.05); IgM mRNA expression was elevated at 6 h in spleen and 6-12 h in head kidney (P < 0.05) then decreased to levels below controls (P < 0.05). These results suggest exposure to 5 mg/L ammonia-N could damage the histological structure of spleen, diminish lysozyme and component C3 serum content, and suppress C3 and IgM expression in spleen and head kidney.

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