gga-mir-133a-3p Regulates Myoblasts Proliferation and Differentiation by Targeting PRRX1

文献类型： 外文期刊

第一作者： Guo, Lijin

作者： Guo, Lijin;Huang, Weiling;Chen, Biao;Bekele, Endashaw Jebessa;Chen, Xiaolan;Cai, Bolin;Nie, Qinghua;Guo, Lijin;Huang, Weiling;Chen, Biao;Bekele, Endashaw Jebessa;Chen, Xiaolan;Cai, Bolin;Nie, Qinghua;Guo, Lijin;Huang, Weiling;Chen, Biao;Bekele, Endashaw Jebessa;Chen, Xiaolan;Cai, Bolin;Nie, Qinghua

作者机构：

关键词： gga-mir-133a-3p; PRRX1; myoblast; proliferation; differentiation

期刊名称：FRONTIERS IN GENETICS （ 影响因子：4.599； 五年影响因子：4.888 ）

ISSN： 1664-8021

年卷期： 2018 年 9 卷

页码：

收录情况： SCI

摘要： Non-coding RNAs play a regulatory role in the growth and development of skeletal muscle. Our previous study suggested that gga-mir-133a-3p was a potential candidate for regulating myoblast proliferation and differentiation in skeletal muscle. The purpose of our study was to reveal the regulatory mechanism of gga-mir-133a-3p in the proliferation and differentiation of chicken myoblasts. Through the detection of cell proliferation activity, cell cycle progression and EdU, we found that gga-mir-133a-3p can significantly inhibit the proliferation of myoblasts. In the process of myogenic differentiation, gga-mir-133a-3p is up-regulated, while gga-mir-133a-3p can significantly promote the up-regulation of differentiation-related muscle-derived factors, indicating that gga-mir-133a-3p can promote the differentiation of myoblasts. Validation at the transcriptional level and protein level proved that gga-mir-133a-3p can inhibit the expression of PRRX1, and the dual-luciferase assay also showed their direct targeting relationship. Correspondingly, PRRX1 can significantly promote myoblast proliferation and inhibit myoblast differentiation. In our study, we confirmed that gga-mir-133a-3p participates in the regulation of proliferation and differentiation of myoblasts by targeting PRRX1.

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