农科机构知识库联盟

Construction and evaluation of HA-epitope-tag introduction onto the VP1 structural protein of a novel HY12 enterovirus

文献类型：外文期刊

第一作者： Liu, Dan

作者： Liu, Dan;Hu, Junying;Li, Xin;Zhang, Qun;Wang, Xinping;Liu, Changming;Hang, Liping;Wei, Yanwu;Zhu, Hongzhen;Wang, Xinping

作者机构：

关键词： HY12 enterovirus; HA-epitope tag; Marker virus; Construction; Evaluation

期刊名称：VIROLOGY （影响因子：3.616；五年影响因子：3.967 ）

ISSN： 0042-6822

年卷期： 2018 年 525 卷

页码：

收录情况： SCI

摘要： In this study, we investigated the feasibility of using enterovirus HY12 as a vector to express an exogenous hemagglutinin (HA)-epitope tag onto the HY12-encoded VP1 protein via a reverse genetic system. Characteristics of recombinant (r) HY12-VP1-HA marker virus were determined by immunoperoxidase mono layer assay, western blot, electron microscopy, and serum-neutralisation assay. Sequence analysis demonstrated that the marker virus stably maintained the HA-epitope-tag in MDBK cells, with no changes in viral morphological features observed relative to those of the parental rHY12 virus. Furthermore, detection by immunofluorescence assay revealed the expression of HA-epitope tag and VP2 protein, which distinguish the marker virus from parental rHY12 virus. In addition, neonatal mice infected with the recombinant marker virus showed various microscopic pathological lesions and generated anti-HY12 virus and -HA-epitope-tag antibodies. These results indicated that the recombinant marker virus represented a valuable platform to promote the development of novel genetic vaccines.

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