Evaluation of the protective efficacy of four newly identified surface proteins of Erysipelothrix rhusiopathiae

文献类型： 外文期刊

第一作者： Zhu, Weifeng

作者： Zhu, Weifeng;Zhu, Weifeng;Wu, Chao;Kang, Chao;Cai, Chengzhi;Wang, Ya;Li, Jingtao;Zhang, Qiang;Sun, Xiaomei;Jin, Meilin;Zhu, Weifeng;Wu, Chao;Kang, Chao;Cai, Chengzhi;Wang, Ya;Li, Jingtao;Jin, Meilin;Wu, Chao;Sun, Xiaomei;Jin, Meilin;Wu, Chao;Jin, Meilin;Zhang, Qiang

作者机构：

关键词： CbpB; Bactericidal; HP0728; HP1472; Protective antigen; GAPDH

期刊名称：VACCINE （ 影响因子：3.641； 五年影响因子：3.816 ）

ISSN： 0264-410X

年卷期： 2018 年 36 卷 52 期

页码：

收录情况： SCI

摘要： Erysipelothrix rhusiopathiae is the causative agent of animal erysipelas and human erysipeloid. Bacterial surface proteins are promising vaccine candidates. We recently identified 3 E. rhusiopathiae surface proteins (GAPDH, HP0728, and HP1472) and characterized their roles as virulence factors. However, their efficacy as protective antigens is still unknown. The N-terminal region of a previously identified surface protein, CbpB (CbpB-N), is speculated to be a protective antigen, but this needs to be verified. The aim of this study was to evaluate the protective efficacy of GAPDH, HP0728, HP1472, and CbpB-N. Immunization with recombinant GAPDH provided complete protection in a mouse model, recombinant CbpB-N provided partial protection, while recombinant HP0728 and HP1472 provided no protection. Recombinant GAPDH also provided good protection in a pig model. GAPDH antiserum exhibited significant blood bactericidal activity against E. rhusiopathiae. In conclusion, GAPDH and CbpB-N were found to be protective antigens of E. rhusiopathiae, and GAPDH is a promising vaccine candidate. (C) 2018 Elsevier Ltd. All rights reserved.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序