Systematic Identification and Analysis of Lysine Succinylation in Strawberry Stigmata

文献类型： 外文期刊

第一作者： Fang, Xianping

作者： Fang, Xianping;Yang, Jian;Chen, Jianping;Fang, Xianping;Jiang, Aili;Xi, Xiaojun;Zha, Qian;Zhang, Liqing;Xin, Ya;Liu, Hui;Sheng, Zheliang;Wang, Fang;Yan, Chengqi;Dai, Liangying

作者机构：

关键词： strawberry stigmata; succinylation; proteomics; AP2; clathrin

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN： 0021-8561

年卷期： 2018 年 66 卷 50 期

页码：

收录情况： SCI

摘要： The various post-translational modifications (PTMs) of plant proteins have important regulatory roles in development. We therefore examined various modified proteins from strawberry stigmata and found that succinylation of lysine residues was the most abundant type of modification. We then subjected proteins from strawberry stigmata to an efficient enrichment method for succinylated peptides and identified 200 uniquely succinylated lysines (Suks) in 116 proteins. A bioinformatics analysis revealed that these proteins are involved in important biological processes, including stress responses, vesicular transport, and energy metabolism. Proteomics, combined with immunoprecipitation and immunoblotting, revealed an obvious increase in succinylation of the assembly polypeptide 2 (AP2) and clathrin from 0.5 to 2 h after pollination, suggesting that succinylation is involved in the recognition of pollen-stigma signaling substances and vesicular transport. These results suggest that AP2/clathrin-mediated vesicular transport processes are regulated by lysine succinylation during pollen recognition.

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