Functional Analysis of Three Rice Chloroplast Transit Peptides

文献类型： 外文期刊

第一作者： He Lei

作者： He Lei;Chen Guang;Zhang Sen;Qiu Zhennan;Hu Jiang;Zeng Dali;Zhang Guangheng;Dong Guojun;Gao Zhenyu;Ren Deyong;Shen Lan;Guo Longbiao;Qian Qian;Zhu Li

作者机构：

关键词： rice; chloroplast transit peptide; translocated function; subcellular location

期刊名称：RICE SCIENCE （ 影响因子：3.333； 五年影响因子：4.226 ）

ISSN： 1672-6308

年卷期： 2019 年 26 卷 1 期

页码：

收录情况： SCI

摘要： Chloroplast transit peptides (CTPs) can be used to transport non-chloroplastic proteins into the chloroplasts. Here, we studied the CTPs of three rice (Oryza sativa L.) chloroplast-localized proteins and found that their CTPs could be used to transport non-chloroplast-localized proteins into the chloroplasts. Fusion proteins lacking the CTP remained located in the cytoplasm. Furthermore, we constructed green fluorescent protein fusion vectors with the three CTPs and three non-chloroplast-localized proteins, Ghd10, MULTI-FLORET SPIKELET1 (MFS1), and SHORTENED UPPERMOST INTERNODE 1 (SUI1). After transforming these constructs into rice protoplasts, the fusion proteins all localized in the chloroplasts. Collectively, our results showed that these CTPs can transport non-chloroplast-localized proteins into the chloroplasts, and more importantly, these CTPs can be applied to engineer chloroplast metabolism.

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