Simultaneous Determination of N-epsilon-(carboxymethyl) Lysine and N-epsilon-(carboxyethyl) Lysine in Different Sections of Antler Velvet after Various Processing Methods by UPLC-MS/MS

文献类型： 外文期刊

第一作者： Gong, Rui-ze

作者： Gong, Rui-ze;Wang, Yan-hua;Wang, Yu-fang;Chen, Bao;Gao, Kun;Sun, Yin-shi;Wang, Yan-hua;Chen, Bao;Gao, Kun

作者机构：

关键词： advanced glycation end-products (AGEs); N-epsilon-(carboxymethyl) lysine (CML); N-epsilon-(carboxyethyl) lysine (CEL); antler velvet processing; UPLC-MS/MS

期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）

ISSN： 1420-3049

年卷期： 2018 年 23 卷 12 期

页码：

收录情况： SCI

摘要： N-epsilon-(Carboxymethyl) lysine (CML) and N-epsilon-(carboxyethyl) advanced glycation end-products (AGEs) and are frequently used as markers of AGE formation. AGEs, such as CML and CEL, have harmful effects in the human body and have been closely linked to many diseases such as diabetes and uremia. However, details on the contents of CML and CEL after applying different antler velvet processing methods are lacking. In this research, a robust lysine (CEL) are two typical UPLC-MS/MS method has been developed for the simultaneous determination of CML and CEL in various sections of antler velvet processed with different methods. In addition, factors affecting the CML and CEL contents are discussed. The CML contents of antler velvet after freeze-drying, boiling, processing without blood, and processing with blood were 74.55-458.59, 119.44-570.69, 75.36-234.92, and 117.11-456.01 g/g protein, respectively; the CEL contents were 0.74-12.66, 11.33-35.93, 0.00-6.75, and 0.00-23.41 g/g protein, respectively. The different contents of CML and CEL in the different samples of antler velvet result from the different interactions of the protein and lysine at different temperatures. These data can be used to estimate the potential consumer intake of CML and CEL from antler velvet and for guiding producers on how to reduce the production of CML and CEL.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序