Ginseng-DF ameliorates intestinal mucosal barrier injury and enhances immunity in immunosuppressed mice by regulating MAPK/NF-κB signaling pathways

文献类型： 外文期刊

第一作者： Sha, Ji-yue

作者： Sha, Ji-yue;Liu, Zheng-bo;Lu, Yu-shun;Qu, Di;Sun, Yin-shi;Chen, Ke-Cheng;Li, Wei;Liu, Shuang;Ma, Jian-kai

作者机构：

关键词： Ginseng-DF; CTX; Immunosuppression; Intestinal mucosal barrier injury; MAPK/NF-kappa B signaling pathway

期刊名称：EUROPEAN JOURNAL OF NUTRITION （ 影响因子：5.0； 五年影响因子：5.2 ）

ISSN： 1436-6207

年卷期： 2024 年

页码：

收录情况： SCI

摘要： PurposeDietary fiber (DF) has a good application prospect in effectively restoring the integrity of the intestinal mucosal barrier. Ginseng-DF has good physicochemical properties and physiological activity and shows positive effects in enhancing immunity. The aim of this study was to investigate the protective effect of Ginseng-DF on intestinal mucosal barrier injury induced by cyclophosphamide (CTX) in immunosuppressed mice and its possible mechanism.MethodsThe effects of Gginseng-DF on immune function in mice were studied by delayed-type hypersensitivy, lymphocyte proliferation assay and NK cytotoxicity assay, the T lymphocyte differentiation and intestinal barrier integrity were analyzed by flow cytometry and western blot.ResultsGinseng-DF (2.5% and 5%) could attenuate the inhibition of DTH response by CTX, promote the transformation and proliferation of lymphocytes, and stimulate NK effector cell activity. At the same time, Ginseng-DF could restore the proportion of CD4+/CD8+ T lymphocytes induced by CTX to different extents, improved spleen tissue damage, promoted the secretion of immunoglobulin IgG, and enhanced body immunity. More importantly, Ginseng-DF could up-regulate the contents of TNF-alpha, IFN-gamma, IL-6 and IL-1 beta in serum and intestine of immunosuppressed mice to maintain the balance between Th1/Th2 cytokines, and improve the permeability of intestinal mucosal barrier. Meanwhile, Ginseng-DF could reduce intestinal epithelial cell apoptosis and improve intestinal adaptive immunity in CTX-induced immunosuppressed mice by regulating MAPK/NF-kappa B signaling pathway.ConclusionGinseng-DF can be used as a safe dietary supplement to enhance body immunity and reduce intestinal mucosal injury caused by CTX.

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