Construction of a high-density genetic linkage map and QTL mapping of oleic acid content and three agronomic traits in sunflower (Helianthus annuus L.) using specific-locus amplified fragment sequencing (SLAF-seq)

文献类型： 外文期刊

第一作者： Zhou, Fei

作者： Zhou, Fei;Liu, Yan;Liu, Xuemei;Zhou, Fei;Liu, Yan;Liang, Chunbo;Wang, Wenjun;Li, Cen;Guo, Yongli;Ma, Jun;Yu, Ying;Fan, Lijuan;Yao, Yubo;Zhao, Dongsheng;Huang, Xutang

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关键词： Helianthus annuus L.; high-density genetic map; SLAF-seq; SNP; oleic acid content; agronomic traits; QTL

期刊名称：BREEDING SCIENCE （ 影响因子：2.086； 五年影响因子：2.632 ）

ISSN： 1344-7610

年卷期： 2018 年 68 卷 5 期

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收录情况： SCI

摘要： High-density genetic linkage maps are particularly important for quantitative trait loci (QTL) mapping, genome assembly, and marker-assisted selection (MAS) in plants. In this study, a high-density genetic linkage map of sunflower (Helianthus annuus L.) was constructed using an F-2 population generated from a cross between Helianthus annuus L. '86-1' and 'L-1-OL-1' via specific-locus amplified fragment sequencing (SLAF-seq). After sequence preprocessing, 530.50 M reads (105.60 Gb) were obtained that contained a total of 343,197 SLAFs, of which 39,589 were polymorphic. Of the polymorphic SLAFs, 6,136 were organized into a linkage map consisting of 17 linkage groups (LGs) spanning 2,221.86 cM. with an average genetic distance of 0.36 cM between SLAFs. Based on this high-density genetic map. QTL analysis was performed that focused on four sunflower phenotypic traits: oleic acid content (OAC), plant height (PH), head diameter (HD), and stem diameter (SD). Subsequently, for these four traits eight QTLs were detected that will likely be useful for increasing our understanding of genetic factors underlying these traits and for use in marker-assisted selection (MAS) for future sunflower breeding.

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