Development of a new RT-PCR with multiple primers for detecting Southern African Territories foot-and-mouth disease viruses

文献类型： 外文期刊

第一作者： Liu, Ya-Li

作者： Liu, Ya-Li;Ding, Yao-Zhong;Dai, Jun-Fei;Ma, Bing;He, Ji-Jun;Ma, Wei-Min;Lv, Jian-Liang;Ma, Xiao-Yuan;Ou, Yun-Wen;Wang, Jun;Liu, Yong-Sheng;Chang, Hui-Yun;Wang, Yong-Lu;Zhang, Qiang;Liu, Xiang-Tao;Zhang, Yong-Guang;Zhang, Jie;Liu, Ya-Li;Ding, Yao-Zhong;Dai, Jun-Fei;Ma, Bing;He, Ji-Jun;Ma, Wei-Min;Lv, Jian-Liang;Ma, Xiao-Yuan;Ou, Yun-Wen;Wang, Jun;Liu, Yong-Sheng;Chang, Hui-Yun;Wang, Yong-Lu;Zhang, Qiang;Liu, Xiang-Tao;Zhang, Yong-Guang;Zhang, Jie

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关键词： Southern African Territory foot-and-mouth disease; RT-PCR; diagnosis; specificity; sensitivity

期刊名称：JOURNAL OF VETERINARY RESEARCH （ 影响因子：1.744； 五年影响因子：1.838 ）

ISSN： 2450-7393

年卷期： 2018 年 62 卷 4 期

页码：

收录情况： SCI

摘要： Introduction: The extremely high genetic variation and the continuously emerging variants of foot-and-mouth disease virus (FMDV) of Southern African Territory (SAT) serotypes including SAT1, SAT2, and SAT3 make it necessary to develop a new RT-PCR for general use for monitoring viruses based on the updated genome information. Material and Methods: A FMDV SAT-D8 one-step RT-PCR was established based on the 1D2A2B genes of the SAT serotype viruses with a multiplex primer set. FMDV A, O, C, and Asia 1 serotypes, other vesicular disease viruses, inactivated SAT viruses, and 125 bovine, ovine, caprine and porcine tissue samples collected from the Chinese mainland were included for evaluating the assay. Results: The new RT-PCR was proven to be specific without cross-reactions with Eurasian FMDV, swine vesicular disease virus (SVDV), Seneca valley virus (SVV), or other common viral pathogens of cattle, sheep, goat, and pig. An around 257 bp-sized amplicon clearly appeared when the inactivated SAT viruses were detected. However, all 125 samples collected from FMDV-susceptible animals from the Chinese mainland which has not known SAT epidemics showed negative results. Conclusions: A FMDV SAT-D8 one-step RT-PCR is a promising method for primary screening for FMDV SAT serotypes.

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