Molecular characterization of Na+/K+/2Cl(-) cotransporter 1 alpha from Trachinotus ovatus (Linnaeus, 1758) and its expression responses to acute salinity stress

文献类型： 外文期刊

第一作者： Zhao, Chao-Ping

作者： Zhao, Chao-Ping;Guo, Hua-Yang;Zhu, Ke-Cheng;Guo, Liang;Zhang, Nan;Liu, Bao-Suo;Yang, Jing-Wen;Liu, Bo;Jiang, Shi-gui;Zhang, Dian-Chang;Zhao, Chao-Ping;Liu, Bo;Zhang, Dian-Chang;Guo, Hua-Yang;Zhu, Ke-Cheng;Guo, Liang;Zhang, Nan;Liu, Bao-Suo;Yang, Jing-Wen;Jiang, Shi-gui;Zhang, Dian-Chang;Guo, Hua-Yang;Zhu, Ke-Cheng;Guo, Liang;Zhang, Nan;Liu, Bao-Suo;Yang, Jing-Wen;Jiang, Shi-gui;Zhang, Dian-Chang

作者机构：

关键词： Trachinotus ovetus; nkcc1a; Genomic structure; Salinity; Osmoregulation

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY （ 影响因子：2.231； 五年影响因子：2.215 ）

ISSN： 1096-4959

年卷期： 2018 年 223 卷

页码：

收录情况： SCI

摘要： Trachinotus ovatus is widely cultured in the ponds and gulf on the southeast coast of China. The dramatic salinity decrease caused by heavy rainfall could cause mass mortality of T. ovatus in aquaculture. It is very important to understand the osmoregulatory mechanism of T. ovatus. Na+/K+/2Cl(-) cotransporter la (NKCC1a) is involved in the osmoregulation of fish and plays a crucial role in cell volume homeostasis and maintenance of the electrolyte content. In this study, we characterized nkcc1a (designed as Tonkcc1a) from T. ovatus and investigated its expression responses to acute salinity changes. Tonkcc1a is approximately 70 kb in length and contains 26 exons and 25 introns. The phylogenetic analysis confirmed that ToNKCC1a belonged to the NKCC1a subclade. Quantitative real-time (qRT-PCR) analysis indicated that Tonkcc1a was ubiquitously expressed in all examined tissues, with the highest mRNA levels observed in gills, and the lowest level in liver. When T. ovatus were transferred from seawater (30 parts per thousand) into fresh water, the expression levels of Tonkcc1a mRNA were significantly downregulated in gills and kidney, whereas its expression level was markedly upregulated in intestine. When transferred from seawater (30 parts per thousand) to 10 parts per thousand sea water, the expression levels of Tonkcc1a mRNA were clearly increased in gills and kidney. When transferred from seawater (30 parts per thousand) to 20 parts per thousand sea water, the expression of Tonkcc1a mRNA increased to some extent in gills, kidney, and intestine. When transferred from seawater (30 parts per thousand) to 40 parts per thousand sea water, the expression levels of Tonkcc1a mRNA were dramatically upregulated in gills and intestine compared to that in the control. These results suggested that Tonkcc1a was involved in the response to acute salinity changes.

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