Discovery and characterization of a novel picorna-like RNA virus in the cotton bollworm Helicoverpa armigera

文献类型： 外文期刊

第一作者： Yuan, He

作者： Yuan, He;Wu, Kongming;Xu, Pengjun;Graham, Robert I.;Wilson, Kenneth

作者机构：

关键词： Helicoverpa armigera; Cotton bollworm; Picoma-like viruses; Transmission strategy; Covert virus

期刊名称：JOURNAL OF INVERTEBRATE PATHOLOGY （ 影响因子：2.841； 五年影响因子：3.368 ）

ISSN： 0022-2011

年卷期： 2019 年 160 卷

页码：

收录情况： SCI

摘要： We characterize a novel picorna-like virus, named Helicoverpa armigera Nora virus (HaNV), with a genome length of 11,200 nts, the sequence of which was isolated from the lepidopteran host cotton bollworm Helicoverpa armigera, using RNA-Seq. Phylogenetic analysis, using the putative amino acid sequence of the conserved RNA-dependent RNA polymerase (RdRp) domain, indicated that HaNV clustered with Spodoptera exigua Nora virus, Drosophila Nora virus and Nasonia vitripennis virus-3 with a high bootstrap value (100%), which might indicate a new viral family within the order Picornavirales. HaNV was efficiently horizontally transmitted between hosts via contaminated food, and transmission was found to be dose-dependent (up to 100% efficiency with 10(9) viral copy number/mu l). HaNV was also found to be transmitted vertically from parent to offspring, mainly through transovum transmission (virus contamination on the surface of the eggs), but having a lower transmission efficiency (around 43%). Infection distribution within the host was also investigated, with HaNV mainly found in only the gut of both adult moths and larvae (> 90%). Moreover, our results showed that HaNV appears not to be an overtly pathogenic virus to its host.

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