Three-phase partitioning for the direct extraction and separation of bioactive exopolysaccharides from the cultured broth of Phellinus baumii
文献类型： 外文期刊
第一作者： Wang, Yao-Yao
作者： Wang, Yao-Yao;Ma, Haile;Ding, Zhi-Chao;Yan, Jing-Kun;Yang, Yan;Wang, Wen-Han;Zhang, He-Nan;Yang, Yan;Wang, Wen-Han;Zhang, He-Nan
作者机构：
关键词： Phellinus baumii; Exopolysaccharide; Three-phase partitioning
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）
ISSN： 0141-8130
年卷期： 2019 年 123 卷
页码：
收录情况： SCI
摘要： In this study, three-phase partitioning (TPP) was used to directly extract and separate bioactive exopolysaccharides (EPSs) from a cultured broth of Phellinus baumii. The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t-butanol, 30 min, and 35 degrees C. A multifrequency power ultrasound in a sequential mode coupled with TPP resulted in similar to 9.12% increment in extraction yield and similar to 80% reduction in extraction time compared with those of traditional TPP. The carbohydrate (88.21%) and uronic acid (3.37%) contents of partially purified EPS were higher than those of EPS-C obtained through conventional ethanol precipitation and separation methods. EPS and EPS-C exhibited similar preliminary structural characteristics and different monosaccharide compositions and molecular weights. The radical-scavenging abilities, antioxidant capacities, alpha-amylase and alpha-glycosidase inhibitory activities, and macrophage stimulation activities of EPS were also higher than those of EPS-C. Therefore, it could be concluded that TPP as a simple and green separation technique could be used to directly extract and separate bioactive EPS from the fermentation broths of mushrooms and other fungi. (C) 2018 Published by Elsevier B.V.
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