Engagement of cellular cholesterol in the life cycle of classical swine fever virus: its potential as an antiviral target

文献类型： 外文期刊

第一作者： Yu, Shaoxiong

作者： Yu, Shaoxiong;Yin, Caixia;Song, Kun;Li, Su;Zheng, Guang-Lai;Li, Lian-Feng;Wang, Jinghan;Li, Yongfeng;Luo, Yuzi;Sun, Yuan;Qiu, Hua-Ji

作者机构：

关键词： classical swine fever virus; cellular cholesterol; antiviral target; internalization; 25-hydroxycholesterol

期刊名称：JOURNAL OF GENERAL VIROLOGY （ 影响因子：3.891； 五年影响因子：3.719 ）

ISSN： 0022-1317

年卷期： 2019 年 100 卷 2 期

页码：

收录情况： SCI

摘要： Classical swine fever virus (CSFV), the etiological agent of classical swine fever in pigs, is a member of the Pestivirus genus within the Flaviviridae family. It has been proposed that CSFV infection is significantly inhibited by methyl-beta-cyclodextrin (M beta CD) treatment. However, the exact engagement of cellular cholesterol in the life cycle of CSFV remains unclear. Here, we demonstrated that pretreatment of PK-15 cells with M beta CD significantly decreased the cellular cholesterol level and resulted in the inhibition of CSFV infection, while replenishment of exogenous cholesterol in M beta CD-treated cells recovered the cellular cholesterol level and restored the viral infection. Moreover, we found that depletion of cholesterol acted on the early stage of CSFV infection and blocked its internalization into the host cells. Furthermore, we showed that 25-hydroxycholesterol, a regulator of cellular cholesterol biosynthesis, exhibited a potent anti-CSFV activity by reducing cellular cholesterol level. Taken together, our findings highlight the engagement of cholesterol in the life cycle of CSFV and its potential use as an antiviral target.

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