Whirly1 enhances tolerance to chilling stress in tomato via protection of photosystem II and regulation of starch degradation

文献类型： 外文期刊

第一作者： Zhuang, Kunyang

作者： Zhuang, Kunyang;Kong, Fanying;Zhang, Song;Yang, Minmin;Liu, Zhuangbin;Wang, Yong;Ma, Nana;Meng, Qingwei;Meng, Chen

作者机构：

关键词： chilling stress; D1; photosynthesis; SlWHY1; starch; tomato

期刊名称：NEW PHYTOLOGIST （ 影响因子：10.151； 五年影响因子：10.475 ）

ISSN： 0028-646X

年卷期： 2019 年 221 卷 4 期

页码：

收录情况： SCI

摘要： In plants, the chilling response involves decreased photosynthetic capacity and increased starch accumulation in chloroplasts. However, the mechanisms that modulate these processes remain unclear. We found that the SlWHY1 gene is significantly induced by chilling stress (4 degrees C) in tomato. Three SlWHY1 overexpression (OE) lines grew better than the wild type (WT) under chilling stress; the OE plants retained intact photosynthetic grana lamellae and showed enhanced hydrolysis of starch. By contrast, RNAi lines that inhibited SlWHY1 were more affected than the corresponding WT cultivar. Their grana lamellae were damaged and starch content increased. The psbA gene encodes the key photosystem II (PSII) protein D1. We show that SlWHY1 binds to the upstream region (A/GTTACCCT/A) of SlpsbA and enhances the de novo synthesis of D1 in chloroplasts. Additionally, SlWHY1 regulates the expression of the starch-degrading enzyme alpha-amylase (SlAMY3-L) and the starch synthesis-related enzyme isoamylase gene (SlISA2) in the nucleus, thus modulating the starch content in chloroplasts. We demonstrate that SlWHY1 enhances the resistance of tomato to chilling stress by maintaining the function of PSII and degrading starch. Thus, overexpression of WHY1 may be an effective strategy for enhancing resistance to chilling stress of chilling-sensitive crops in agricultural production.

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