Factors affecting hydrolytic action of xylanase during pennisetum saccharification: Role of cellulose and its derivatives

文献类型： 外文期刊

第一作者： Chen, Xiang

作者： Chen, Xiang;Xin, Donglin;Wang, Rui;Qin, Yujie;Wen, Peiyao;Zhang, Junhua;Hou, Xincun

作者机构：

关键词： Xylanase; Xylosidase; Inhibition; Adsorption; Cellobiose; Glucose

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：5.645； 五年影响因子：5.749 ）

ISSN： 0926-6690

年卷期： 2019 年 130 卷

页码：

收录情况： SCI

摘要： The effects of cellobiose and glucose as product inhibitors on the hydrolysis of lignocelluloses by cellulase has been confirmed, but the effect of these species on xylanase is still unknown. In this work, the inhibitory effects of cellulose and its derivatives on xylanase in the hydrolysis of Chinese pennisetum, a potential lignocellulosic biomass for the production of biofuels, was investigated. It was found that cellulose, cellobiose, and glucose all have negative effects on the hydrolytic action of endo-xylanase but not on that of beta-xylosidase. Among these inhibitors, cellobiose exhibited the strongest inhibitory effect on xylanase, followed by glucose and cellulose. Cellulose (50 mg/mL), cellobiose (50 mg/mL), and glucose (50 mg/mL) decreased the hydrolysis yields of xylan by 10.4%, 19.7%, and 17.7%, respectively. Kinetic analysis revealed that both cellobiose and glucose competitively inhibited the activity of xylanase. The conclusions of this work help us further understand the inhibitory effects of cellulose derivatives on the hydrolysis of lignocelluloses.

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