文献类型： 外文期刊

第一作者： Zhou Liqing

作者： Zhou Liqing;Wu Biao;Sun Xiujun;Zhao Qing;Zhang Gaowei;Liu Zhihong;Yang Aiguo;Wang Xuemei;Zhou Liqing;Wu Biao;Sun Xiujun;Liu Zhihong;Yang Aiguo

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关键词： Blepharipoda liberate; chromosome; karyotype; lampbrush chromosome

期刊名称：JOURNAL OF OCEANOLOGY AND LIMNOLOGY （ 影响因子：1.265； 五年影响因子：1.276 ）

ISSN： 2096-5508

年卷期： 2019 年 37 卷 1 期

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收录情况： SCI

摘要： Blepharipoda liberate Shen is a commercially valuable seafood species that has important ecological significance in Shandong Province, China. Although B. liberate is crustacean, its external characteristics are not entirely those of shrimps or crabs. The question of whether B. liberate is a shrimp or a crab has been debated in recent years. We studied the karyotype of B. liberate by light microscopy using air-drying and spreading methods. We obtained mitotic chromosomal plates from B. liberate larvae, and from adult B. liberate females subsequent to egg-laying. The results revealed that B. liberate has 53 pairs of chromosomes (i.e., n=53 and 2n=106), a characteristic shared with four species of crab. The karyogram of B. liberate consists of 25 metacentric, 14 submetacentric, 11 subtelocentric and 3 telocentric pairs. We did not find any heteromorphosis sex chromosomes. Tissue from larvae, gills and ovaries can be used for chromosomal investigations, and we found similar lampbrush chromosomes in ovary cells. Comparatively speaking, larvae tissue is more practical, and ovary tissue is more suitable for the preparation of lampbrush chromosomes. B. liberate is more closely related to crabs than to shrimps, based on the numbers of chromosomes. The B. liberate karyotype reported here provides a basis for further comparative cytogenetic studies of species populations.

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