Effect of Gpx3 gene silencing by siRNA on apoptosis and autophagy in chicken cardiomyocytes

文献类型： 外文期刊

第一作者： Gong, Yafan

作者： Gong, Yafan;Yang, Jie;Cai, Jingzeng;Liu, Qi;Zhang, Ziwei;Zhang, Jun Min;Zhang, Ziwei;Zhang, Ziwei

作者机构：

关键词： apoptosis; autophagy; cardiomyocytes; chickens; glutathione peroxidase 3

期刊名称：JOURNAL OF CELLULAR PHYSIOLOGY （ 影响因子：6.384； 五年影响因子：5.987 ）

ISSN： 0021-9541

年卷期： 2019 年 234 卷 6 期

页码：

收录情况： SCI

摘要： Glutathione peroxidase 3 (Gpx3), as an important selenoprotein, is the most crucial antioxidant defense in cardiomyocytes. However, the role of Gpx3 in Se-deficient cardiomyocyte damage still less reported. Here, we developed Gpx3 silence cardiomyocytes culture model (small interfering RNA; siRNA) for research the crosstalk between autophagy and apoptosis. Quantitative real-time PCR and western blot analysis are performed to detect the expression of apoptosis and autophagy-related genes. MDC stain, flow cytometry, AO/EB stain, and electron microscope were performed to observe the changes of cell morphology. Our results reveal that Gpx3 suppression can significant increases in ROS (p < 0.05) levels, which further induced apoptosis through upregulated the expression of Caspase-3 in cardiomyocytes. Meanwhile, we also found that the whole process is accompanied by the occurrence of autophagy, which are promoted by inhibiting the mTOR, and increasing the expression of ATG-7, ATG-10, and ATG-12. Altogether, we conclude that the apoptotic and autophagic response machineries share antagonistic function in Gpx3 knockdown cardiomyocytes.

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