Enzymatic synthesis of 1-caffeoylglycerol with deep eutectic solvent under continuous microflow conditions

文献类型： 外文期刊

第一作者： Liu, Xi

作者： Liu, Xi;Meng, Xiang-Yun;Xu, Yan;Zhang, Dong-Yang;Guan, Hui-Xiang;Wang, Jun;Xu, Yan;Zhang, Dong-Yang;Wang, Jun;Dong, Tao;Zhuang, Yu

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关键词： Biocatalysis; 1-Caffeoylglycerol; Deep eutectic solvent; Kinetics; Microreactor; Mass transfer coefficient

期刊名称：BIOCHEMICAL ENGINEERING JOURNAL （ 影响因子：3.978； 五年影响因子：4.084 ）

ISSN： 1369-703X

年卷期： 2019 年 142 卷

页码：

收录情况： SCI

摘要： 1-caffeoylglycerol (1-CG) is a hydrophilic ester with a high solubility, and has a strong activity to prevent skin cancer caused by ultraviolet. However, the current preparation process to synthesize 1-CG via transesterification from methyl caffeate (MC) and glycerin was not economic due to long reaction time or adding extra field. Therefore, an enzymatic synthesis with deep eutectic solvent chloride-urea (ChCI-urea) in a microreactor was firstly applied under continuous microflow condition to improve the productivity. A maximum 1-CG yield of 96.49% was obtained under the optimized conditions: temperature of 65 degrees C, flow rate of 2 mu L/min, MC concentration of 50 g/L. Compared to the batch reactor, the reaction time reduced by 75%, the K-m value decreased by 5/6, the reuse times of lipase increased by 2.29-fold, the external mass and transfer coefficients increased 10 times, which process was verified by numerical simulation. In addition, the produced 1-CG of 2 mg/mL has a high UV damage remediation ability of 75.16% using HaCaT cell model, which reveals 1-CG can repair the damage of cells radiated by the UV. Thus, this approach represents a convenient and cost-saving method to produce 1-CG using microfluidic biocatalysis.

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