A recombinant adenovirus expressing the E protein of duck Tembusu virus induces protective immunity in duck

文献类型： 外文期刊

第一作者： Tang, Jingyu

作者： Tang, Jingyu;Yin, Dongdong;Wang, Rui;Zhou, Qi;Zhou, Xiaoya;Xing, Xue;Liu, Hong-Mei;Wang, Guijun;Tang, Jingyu;Wang, Rui;Liu, Guangqing;Yin, Dongdong;Liu, Hong-Mei;Wang, Guijun

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关键词： duck Tembusu virus; E protein; experimental infection; protection; recombinant adenovirus

期刊名称：JOURNAL OF VETERINARY MEDICAL SCIENCE （ 影响因子：1.267； 五年影响因子：1.348 ）

ISSN： 0916-7250

年卷期： 2019 年 81 卷 2 期

页码：

收录情况： SCI

摘要： Duck Tembusu virus disease, caused by the duck Tembusu virus (DTMUV), can lead to a severe reduction in egg production and growth retardation in laying ducks and ducklings, respectively. In this study, we engineered a novel recombinant adenovirus expressing the E protein of DTMUV (rAd-E) in AAV-293 cells (analyzed by western blot and indirect immunofluorescence assays). Intramuscular immunization of Cherry Valley ducks with rAd-E was performed to evaluate host cellular and humoral immune responses. Compared to the phosphate-buffered saline administered group and the negative control wild-type adenovirus (wtAd) group, the rAd-E vaccinated group showed increased cellular and humoral responses. The results from the cytokine release and lymphocyte proliferation assays showed that rAd-E induced a stronger cellular immune response than the control group (P< 0.01), 4 weeks after primary immunization. The results of enzyme-linked immunosorbent and virus neutralization assays showed that rAd-E induced higher titers of specific neutralizing antibodies, 2 weeks after primary immunization. The DTMUV challenge experiment showed a higher survival rate (80%) of ducks in the rAd-E group, when challenged with 0.5 ml (ELD50= 10-2.67/0.2 ml) of the DTMUV strain AH-F10. These results indicate that rAd-E effectively protects ducks against DTMUV infection. Therefore, rAd-E could be a vaccine candidate to provide an effective and safe method for prevention and control of DTMUV infection.

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