Clonal seeds from hybrid rice by simultaneous genome engineering of meiosis and fertilization genes

文献类型： 外文期刊

第一作者： Wang, Chun

作者： Wang, Chun;Liu, Qing;Hua, Yufeng;Wang, Junjie;Lin, Jianrong;Wu, Mingguo;Sun, Tingting;Wang, Kejian;Shen, Yi;Cheng, Zhukuan;Mercier, Raphael

作者机构：

期刊名称：NATURE BIOTECHNOLOGY （ 影响因子：54.908； 五年影响因子：50.516 ）

ISSN： 1087-0156

年卷期： 2019 年 37 卷 3 期

页码：

收录情况： SCI

摘要： Heterosis, or hybrid vigor, is exploited by breeders to produce elite high-yielding crop lines, but beneficial phenotypes are lost in subsequent generations owing to genetic segregation. Clonal propagation through seeds would enable self-propagation of F-1 hybrids. Here we report a strategy to enable clonal reproduction of F-1 rice hybrids through seeds. We fixed the heterozygosity of F-1 hybrid rice by multiplex CRISPR-Cas9 genome editing of the REC8, PAIR1 and OSD1 meiotic genes to produce clonal diploid gametes and tetraploid seeds. Next, we demonstrated that editing the MATRILINEAL (MTL) gene (involved in fertilization) could induce formation of haploid seeds in hybrid rice. Finally, we combined fixation of heterozygosity and haploid induction by simultaneous editing of all four genes (REC8, PAIR1, OSD1 and MTL) in hybrid rice and obtained plants that could propagate clonally through seeds. Application of our method may enable self-propagation of a broad range of elite F-1 hybrid crops.

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