Molecular characterization and overexpression of mnp6 and vp3 from Pleurotus ostreatus revealed their involvement in biodegradation of cotton stalk lignin

文献类型： 外文期刊

第一作者： Wang, Yan

作者： Wang, Yan;Li, Guoqing;Jiao, Xiaoyu;Cheng, Xi;Abdullah, Muhammad;Li, Dahui;Lin, Yi;Cai, Yongping;Li, Guoqing;Nie, Fan

作者机构：

关键词： Pleurotus ostreatus; Class II peroxidase; Bioinformatics; Overexpression; Lignin degradation

期刊名称：BIOLOGY OPEN （ 影响因子：2.422； 五年影响因子：2.647 ）

ISSN： 2046-6390

年卷期： 2019 年 8 卷 2 期

页码：

收录情况： SCI

摘要： Fungal secretory heme peroxidase (Class II POD) plays a significant role in biomass conversion due to its lignin-degrading activity. In this study, genome-wide identification and bioinformatics were performed to analyze Pleurotus ostreatus peroxidases (PoPODs). A total of six manganese peroxidases (MnPs) and three versatile peroxidases (VPs) were obtained. Bioinformatics analysis and qRT-PCR showed that P. ostreatus mnp6 (Pomnp6) and P. ostreatus vp3 (Povp3) could be involved in lignin degradation. Both Pomnp6 and Povp3 transgenetic fungi showed significantly increased lignin degradation of cotton stalks. H-1-NMR revealed that Pomnp6 and Povp3 may preferentially degrade S-lignin in cotton stalks and mainly break beta-O-4' bond linkages and hydroxyl. These results support the possible utility of Pomnp6 and Povp3 in natural straw resources and development of sustainable energy.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序