New stationary phase for hydrophilic interaction chromatography to separate chito-oligosaccharides with degree of polymerization 2-6

文献类型： 外文期刊

第一作者： Zhai, Xingchen

作者： Zhai, Xingchen;Zhao, Haitian;Zhang, Min;Yang, Xin;Sun, Jingming;Dong, Aijun;Zhang, Hua;Wang, Jing;Zhai, Xingchen;Yang, Xin;She, Yongxin;Wang, Jing;Yao, Lei

作者机构：

关键词： Chito-oligosaccharides Hydrophilic interaction liquid chromatography (HILIC); Stationary phase; 3-aminophenylboronic acid

期刊名称：JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES （ 影响因子：3.205； 五年影响因子：3.068 ）

ISSN： 1570-0232

年卷期： 2018 年 1081 卷

页码：

收录情况： SCI

摘要： A new 3-aminophenylboronic acid-functionalized stationary phase based on silica for hydrophilic interaction liquid chromatography (HILIC) was developed and showed great HILIC characteristics on separation for chito-oligosaccharides. The material was synthesized by grafting 3-aminophenylboronic acid group to silica, and it was characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), elemental analysis and thermal gravimetric analysis (TGA). Nucleobases and nucleosides were used to evaluate the retention property and to investigate retention mechanism by the models designed for description of partitioning and surface adsorption through adjusting ratio of water in the mobile phase. Parameters affecting chromatography behavior such as ionic strength, buffer pH and column temperature were also investigated. Results have indicated that the retention mechanism was a combination of partitioning and surface adsorption, and the hydrogen bond seemed to be the main force for the retention behavior. Finally, the new 3-aminophenylboronic acid-functionalized based on silica stationary phase was applied to separate chito-oligosaccharide samples with optimized mobile phase conditions and showed acceptable chromatograms.

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