Development of small molecule biosensors by coupling the recognition of the bacterial allosteric transcription factor with isothermal strand displacement amplification
文献类型： 外文期刊
第一作者： Cao, Jiaqian
作者： Cao, Jiaqian;Liu, Weiwei;Fan, Keqiang;Yang, Keqian;Wang, Weishan;Yao, Yongpeng;Cao, Jiaqian;Liu, Weiwei;Fan, Keqiang;Yang, Keqian;Wang, Weishan;Li, Shanshan;Xiang, Wensheng;Kong, Deming;Yao, Yongpeng;Cao, Jiaqian;Liu, Weiwei
作者机构：
期刊名称：CHEMICAL COMMUNICATIONS （ 影响因子：6.222； 五年影响因子：6.008 ）
ISSN： 1359-7345
年卷期： 2018 年 54 卷 38 期
页码：
收录情况： SCI
摘要： Here, we demonstrate an easy-to-implement and general bio-sensing strategy by coupling the small-molecule recognition of the bacterial allosteric transcription factor (aTF) with isothermal strand displacement amplification (SDA) in vitro. Based on this strategy, we developed two biosensors for the detection of an antiseptic, p-hydroxybenzoic acid, and a disease marker, uric acid, using bacterial aTF HosA and HucR, respectively, highlighting the great potential of this strategy for the development of small-molecule biosensors.
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