Streptomyces adonidis sp. nov., a novel actinomycete strain with antifungal activity isolated from the root of Adonis amurensis Regel

文献类型： 外文期刊

第一作者： Du, Rui

作者： Du, Rui;Bing, Hui;Lu, Wentian;Gao, Congting;Kong, Fanxue;Wang, Hongtu;Shen, Yue;Liu, Chongxi;Xiang, Wensheng;Xiang, Wensheng

作者机构：

关键词： Endophyte; Streptomyces; Polyphasic taxonomy; Antifungal activity; Biosynthetic gene cluster

期刊名称：ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY （ 影响因子：1.8； 五年影响因子：2.3 ）

ISSN： 0003-6072

年卷期： 2025 年 118 卷 4 期

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收录情况： SCI

摘要： A novel endophytic actinomycete with antagonistic activity against Fusarium moniliforme, designated strain NEAU-BLH26(T), was isolated from the root of Adonis amurensis Regel collected at the horticultural experiment station of Northeast Agricultural University, Heilongjiang Province, northeast China. Strain NEAU-BLH26(T) exhibited morphological and chemotaxonomic features of the genus Streptomyces. The diamino acid present in its cell wall was identified as LL-diaminopimelic acid, while galactose detected in whole-cell hydrolysates. The menaquinones were identified as MK-9(H-6), MK-9(H-8), and MK-9(H-4). The phospholipid profile comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, two unidentified phospholipids and two unidentified aminolipids. The major fatty acids were determined to be iso-C-16:0 and C-16:0. The DNA G + C content, based on the genome sequence, was 70.5 mol%. Phylogenetic analyses of 16S rRNA gene and whole genome sequences analyses indicated that strain NEAU-BLH26(T) was most closely related to Streptomyces geranii A301(T), with a 16S rRNA gene sequence similarity of 98.69%. However, the average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values confirmed that strain NEAU-BLH26(T) represents a distinct species. This conclusion was further supported by phenotypic and chemotaxonomic differences between strain NEAU-BLH26(T) and its closest relative. Additionally, the secondary metabolite biosynthetic gene clusters in strain NEAU-BLH26(T) were predicted and analyzed. Based on phenotypic, chemotaxonomic and genotypic evidence, strain NEAU-BLH26(T) is proposed as a novel species of the genus Streptomyces, for which the name Streptomyces adonidis sp. nov. is suggested. The type strain is NEAU-BLH26(T) (= JCM 36414(T) = MCCC 1K08678(T)).

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