GmDRR1, a dirigent protein resistant to Phytophthora sojae in Glycine max (L.) Merr

文献类型： 外文期刊

第一作者： Chen Qing-shan

作者： Chen Qing-shan;Yu Guo-long;Zou Jia-nan;Wang Jing;Qiu Hong-mei;Zhu Rong-sheng;Chang Hui-lin;Jiang Hong-wei;Hu Zhen-bang;Li Chang-yu;Zhang Yan-jiao;Wang Jin-hui;Wang Xue-ding;Gao Shan;Liu Chun-yan;Qi Zhao-ming;Xin Da-wei;Fu Yong-fu

作者机构：

关键词： dirigent domain; GmDRR1; soybean; Phytophthora sojae; oomycete; proteomics

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2018 年 17 卷 6 期

页码：

收录情况： SCI

摘要： Soil-borne pathogen Phytophthora sojae is an oomycete that causes devastating damage to soybean yield. To mine original resistant genes in soybean is an effective and environmentally-friend approach controlling the disease. In this study, soybean proteins were extracted from the first trifoliolates infected by predominant P sojae race 1 and analyzed by two-dimensional gel electrophoresis. Nineteen differently-expressed protein spots were detected, and 10 of them were further applied for Matrix-Assisted Laser Desorption/lonization Time of Flight Mass Spectrometry Assay. One protein containing a dirigent (DIR) domain was identified and belonged to the DIR-b/d family. Therefore, it was named as GmDRR1 (gycine max Disease Resistance Response 1). Then, GmDRR1 gene was pathologically confirmed to be involved in the resistant to P sojae in soybean. GmDRR1-GFP (green fluorescent protein) fusion proteins localized in the cell membrane. qRT-PCR results showed GmDRR1 gene expressed differently in P sojae resistant- and susceptible-soybean cultivars. By the promoter analysis, we found a haplotype H8 was existing in most resistant soybean varieties, while a haplotype H77 was existing in most susceptible soybean varieties. The H77 haplotype had seven SNPs (C to A, G to C, C to A, T to A, T to C, T to C, and T to A) and two single nucleotide insertions. The results supported that the expression difference of GmDRR1 genes between P sojae resistant- and susceptible-soybean cultivars might depend on the GmDRR1 promoter SNPs. The results suggested that GmDRR1 was a dirigent protein involved in soybean resistant to P sojae and paved a novel way for investigation of the molecular regulatory mechanism of the defense response to P sojae in soybean.

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