Molecular cloning and identification of the hatching enzyme gene in Rondotia menciana

文献类型： 外文期刊

第一作者： Tang, Shunming

作者： Tang, Shunming;Wang, Huanying;Wang, Xingkai;Liu, Longshan;Pu, Yuexia;Wang, Xuezheng;Zhao, Qiaoling;Cheng, Jialing;Shen, Xingjia;Tang, Shunming;Wang, Huanying;Wang, Xingkai;Liu, Longshan;Pu, Yuexia;Wang, Xuezheng;Zhao, Qiaoling;Cheng, Jialing;Shen, Xingjia;Wang, Huanying;Pu, Yuexia

作者机构：

关键词： expression pattern; Lepidopteran pests

期刊名称：SCIENCEASIA （ 影响因子：0.615； 五年影响因子：0.708 ）

ISSN： 1513-1874

年卷期： 2018 年 44 卷 4 期

页码：

收录情况： SCI

摘要： Rondotia menciana Moore (Lepidoptera: Bombycidae) is a major pest of mulberry. We cloned the full-length cDNA of the hatching enzyme from R. menciana eggs (RmHE) using the RACE-PCR and SMART cDNA synthesis technique. The cDNA is 1325 bp long and contains an ORF of 885 bp. It encodes 294 amino acid residues, including a putative signal peptide of 16 amino acid residues. Two conserved signature sequences of insect hatching enzyme (HE) harbour in the RmHE. The deduced amino acid sequence of RmHE has 30-84% identity to HE sequences in other insect species, and RmHE is most similar to that of wild silkworm HE. RmHE transcripts could be detected in R. menciana eggs at different developmental stages. Levels remained low during the early stages, increased greatly in 7-d eggs, and reached a maximum in 9-d eggs. Changes in the levels of RmHE transcripts occurred in accordance with the process of embryo development and eclosion. This indicated that RmHE plays an important role in these processes. RmHE transcripts were also detected in larval midguts, suggesting that RmHE is involved in food digestion. As far as we know, this is the first report on an HE gene in Lepidopteran pests and will offer some basic information for developing an efficient control method during the hatching process for agricultural Lepidopteran pests.

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