Effective mucosal live attenuated Salmonella vaccine by deleting phosphotransferase system component genes ptsI and crr

文献类型： 外文期刊

第一作者： Zhi, Yong

作者： Zhi, Yong;Lin, Shun Mei;Jang, A-Yeung;Ahn, Ki Bum;Ji, Hyun Jung;Lim, Sangyong;Seo, Ho Seong;Zhi, Yong;Lim, Sangyong;Seo, Ho Seong;Jang, A-Yeung;Ji, Hyun Jung;Ji, Hyun Jung;Guo, Hui-Chen

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关键词： Salmonella Typhimurium; phosphotransferase system; live attenuation vaccine

期刊名称：JOURNAL OF MICROBIOLOGY （ 影响因子：3.422； 五年影响因子：3.28 ）

ISSN： 1225-8873

年卷期： 2019 年 57 卷 1 期

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收录情况： SCI

摘要： Salmonella enterica is a major human pathogen that causes invasive non-typhoidal Salmonellosis (iNTS), resulting in significant morbidity and mortality. Although a number of pre-clinical and clinical studies have reported on the feasibility of developing a safe and effective vaccine against iNTS, there have been no licensed Salmonella vaccines available to protect against NTS strains. Vaccine formulations of highest priority for NTS are live attenuated vaccines, which can elicit effective induction of intestinal mucosal and intracellular bacteria-specific cell mediated immune responses. Since glucose is crucial for intracellular survival and replication in host cells, we constructed strains with mutations in components of the glucose uptake system, called the phosphotransferase system (PTS), and compared the relative virulence and immune responses in mice. In this study, we found that the strain with mutations in both ptsI and crr (KST0556) was the most attenuated strain among the tested strains, and proved to be highly effective in inducing a mucosal immune response that can protect against NTS infections in mice. Thus, we suggest here that KST0556 (ptsIcrr) is a potential live vaccine candidate for NTS, and may also be a candidate for a live delivery vector for heterologous antigens. Moreover, since PTS is a well-conserved glucose transporter system in both Gramnegative and Gram-positive bacteria, the ptsI and crr genes may be potential targets for creating live bacterial vectors or vaccine strains.

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