Identification of Soybean Genes Related to Soybean Seed Protein Content Based on Quantitative Trait Loci Collinearity Analysis

文献类型： 外文期刊

第一作者： Huang, Shiyu

作者： Huang, Shiyu;Yu, Jingyao;Li, Yingying;Wang, Jingxin;Wang, Xinyu;Qi, Huidong;Xu, Mingyue;Qin, Hongtao;Yin, Zhengong;Mei, Hongyao;Gao, Hongxiu;Liu, Shanshan;Zhang, Zhenguo;Zhu, Rongsheng;Liu, Chunyan;Wu, Xiaoxia;Jiang, Hongwei;Hu, Zhenbang;Xin, Dawei;Chen, Qingshan;Qi, Zhaoming;Zhang, Shuli

作者机构：

关键词： soybean protein QTLs; collinearity analysis; candidate genes; QTL validation; SDS-PAGE analysis; qRT-PCR verification; gene ontology enrichment analysis

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN： 0021-8561

年卷期： 2019 年 67 卷 1 期

页码：

收录情况： SCI

摘要： Increasing the protein content of soybean seeds through a higher ratio of glycinin is important for soybean breeding and food processing; therefore, the integration of different quantitative trait loci (QTLs) is of great significance. In this study, we investigated the collinearity of seed protein QTLs. We identified 192 collinear protein QTLs that formed six hotspot regions. The two most important regions had seed protein 36-10 and seed protein 36-20 as hub nodes. We used a chromosome segment substitution line (CSSL) population for QTL validation and identified six CSSL materials with collinear QTLs. Five materials with higher protein and glycinin contents in comparison to the recurrent parent were analyzed. A total of 13 candidate genes related to seed protein from the QTL hotspot intervals were detected, 8 of which had high expression in mature soybean seeds. These results offer a new analysis method for molecular-assisted selection (MAS) and improvement of soybean product quality.

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