Cellular Response of Escherichia coli to Photocatalysis: Flagellar Assembly Variation and Beyond

文献类型： 外文期刊

第一作者： Zhang, Jingtao

作者： Zhang, Jingtao;Wang, Xueying;Suo, Xinying;Liu, Xing;Yuan, Mingming;Wang, Guanglu;Liu, Bingkun;Shi, Hengzhen;Liang, Chengzhen

作者机构：

关键词： photocatalysis; cellular response; RNA-seq; flagellar assembly; metabolic alteration

期刊名称：ACS NANO （ 影响因子：15.881； 五年影响因子：16.207 ）

ISSN： 1936-0851

年卷期： 2019 年 13 卷 2 期

页码：

收录情况： SCI

摘要： Bacterial cells can be inactivated by external reactive oxygen species (ROS) produced by semiconductor photocatalysis. However, little is known about cellular responses to photocatalysis. For a better understanding of this issue, one strain of Escherichia colt (E. coli, hereafter named as MT), which has an increased ability to metabolize carbon sources, was screened out from the wild-type (WT) E. coli K12 by repeated exposure to photocatalysis with palladium oxide modified nitrogen-doped titanium dioxide. In this study, transcriptome sequencing of the WT and MT strains that were exposed or unexposed to photocatalysis were carried out. Cellular responses to photocatalysis were inferred from the functions of genes whose transcripts were either increased or decreased. Upregulation of expression of bacterial flagellar assembly genes used for chemotaxis was detected in cells exposed to semilethal photocatalytic conditions of the WT E. coli. Increased capability to degrade superoxide radicals and decreased bacterial flagellar assembly and chemotaxis were observed in MT E. coli compared to WT cells. We conclude that the differences in motility and intracellular ROS between MT and WT are directly related to survivability of E. coli during exposure to photodisinfection.

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