MicroRNA-205 affects mouse granulosa cell apoptosis and estradiol synthesis by targeting CREB1

文献类型： 外文期刊

第一作者： Zhang, Pengju

作者： Zhang, Pengju;Lang, Hongyan;Wang, Weixia;Liu, Xiaohui;Liu, Haiyan;Tan, Chengcheng;Li, Xintao;Zhao, Yumin;Wu, Xinghong;Wang, Jun

作者机构：

关键词： apoptosis; cyclic AMP response element (CRE)-binding protein 1 (CREB1); estradiol synthesis; follicular atresia; miR-205

期刊名称：JOURNAL OF CELLULAR BIOCHEMISTRY （ 影响因子：4.429； 五年影响因子：4.266 ）

ISSN： 0730-2312

年卷期： 2019 年 120 卷 5 期

页码：

收录情况： SCI

摘要： MicroRNA-205 (miR-205) is involved in various physiological and pathological processes, but its biological function in follicular atresia remains unclear. In this study, we investigated miR-205 expression in mouse granulosa cells (mGCs) and analyzed its functions in primary mGCs by performing a series of in vitro experiments. Quantitative real-time polymerase chain reaction showed that miR-205 expression was significantly higher in early atretic follicles and progressively atretic follicles than in healthy follicles. miR-205 overexpression in mGCs significantly promoted apoptosis and caspase-3/9 activities, as well as inhibited estrogen (E2) release and cytochrome P450 family 19 subfamily A polypeptide 1 (CYP19A1, a key gene in E2 production) expression. Bioinformatics and luciferase reporter assays revealed that the gene encoding cyclic AMP response element (CRE)-binding protein 1 (CREB1) was a direct target of miR-205 in mGCs. CREB1 upregulation partially rescued the effects of miR-205 on apoptosis, caspase-3/9 activities, E2 production, and CYP19A1 expression on mGCs. These results indicate that miR-205 might play an important role in ovarian follicular development and provide new insights into follicular atresia

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