Elovl4a participates in LC-PUFA biosynthesis and is regulated by PPAR alpha beta in golden pompano Trachinotus ovatus (Linnaeus 1758)

文献类型： 外文期刊

第一作者： Zhu, Ke-Cheng

作者： Zhu, Ke-Cheng;Song, Ling;Guo, Hua-Yang;Guo, Liang;Zhang, Nan;Liu, Bao-Suo;Jiang, Shi-Gui;Zhang, Dian-Chang;Zhu, Ke-Cheng;Guo, Hua-Yang;Guo, Liang;Zhang, Nan;Liu, Bao-Suo;Jiang, Shi-Gui;Zhang, Dian-Chang;Zhu, Ke-Cheng;Guo, Liang;Zhang, Nan;Liu, Bao-Suo;Zhang, Dian-Chang;Jiang, Shi-Gui

作者机构：

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2019 年 9 卷

页码：

收录情况： SCI

摘要： The elongases of very long-chain fatty acids (Elovls) are responsible for the rate-limiting elongation process in long-chain polyunsaturated fatty acid (LC-PUFA) biosynthesis. The transcription factor, PPAR alpha, regulates lipid metabolism in mammals; however, the detailed mechanism whereby PPAR alpha b regulates Elovls remains largely unknown in fish. In the present study, we report the full length cDNA sequence of Trachinotus ovatus Elovl4a (ToElovl4a), which encodes a 320 amino acid polypeptide that possesses five putative membrane-spanning domains, a conserved HXXHH histidine motif and an ER retrieval signal. Phylogenetic analysis revealed that the deduced protein of ToElovl4a is highly conserved with the Oreochromis niloticus corresponding homologue. Moreover, functional characterization by heterologous expression in yeast indicated that ToElovl4a can elongate C18 up to C20 polyunsaturated fatty acids. A nutritional study showed that the protein expressions of ToElovl4a in the brain and liver were not significantly affected among the different treatments. The region from PGL3-basic-Elovl4a-5 (-148 bp to + 258 bp) is defined as the core promoter via a progressive deletion mutation of ToElovl4a. The results from promoter activity assays suggest that ToElovl4a transcription is positively regulated by PPAR alpha b. Mutation analyses indicated that the M2 binding site of PPAR alpha b is functionally important for protein binding, and transcriptional activity of the ToElovl4a promoter significantly decreased after targeted mutation. Furthermore, PPAR alpha b RNA interference reduced ToPPAR alpha b and ToElovl4a expression at the protein levels in a time-dependent manner. In summary, PPAR alpha b may promote the biosynthesis of LC-PUFA by regulating ToElovl4a expression in fish.

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