Purification and Characterization of a Secretory Alkaline Metalloprotease with Highly Potent Antiviral Activity from Serratia marcescens Strain S3
文献类型： 外文期刊
第一作者： Qin, Yuanxia
作者： Qin, Yuanxia;Song, Liyun;Wu, Yuanhua;Qin, Yuanxia;Wang, Jie;Wang, Fenglong;Shen, Lili;Zhou, Haixiang;Sun, Hangjun;Hao, Kaiqiang;Song, Liyun;Yang, Jinguang;Zhou, Zhicheng;Zhang, Chaoqun
作者机构：
关键词： Serratia marcescens; secretory alkaline metalloprotease; Tobacco mosaic virus; antiviral activity
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）
ISSN： 0021-8561
年卷期： 2019 年 67 卷 11 期
页码：
收录情况： SCI
摘要： In this study we report a secretory protein that was purified from Serratia marcescens strain S3 isolated from soil from the tobacco rhizosphere. Subsequent mass spectrometry and annotation characterized the protein as secretory alkaline metalloprotease (SAMP). SAMP plays a crucial role in inhibiting Tobacco mosaic virus (TMV). Transmission electron microscopy (TEM), dynamic light scattering (DLS), confocal microscopy, and microscale thermophoresis (MST) were employed to investigate the anti-TMV mechanism of SAMP. Our results demonstrated that SAMP, as a hydrolytic metal protease, combined and hydrolyzed TMV coat proteins to destroy the virus particles. This study is the first to investigate the antiviral effects of a S. marcescens metalloprotease, and our finding suggests that S. marcescens-S3 may be agronomically useful as a disease-controlling factor active against Tobacco mosaic virus.
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