Identification and localization of SAS-6 in the microsporidium Nosema bombycis

文献类型： 外文期刊

第一作者： Dai, Weijiang

作者： Dai, Weijiang;Li, Nan;Zhang, Zhilin;Chen, Gong;Li, Xiaoliang;Peng, Xiangran;Zhang, Yiling;Xu, Li;Shen, Zhongyuan;Zhang, Yiling;Xu, Li;Shen, Zhongyuan

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关键词： Microsporidia; Nosema bombycis; Spindle plaque; SAS-6; Karyokinesis

期刊名称：INFECTION GENETICS AND EVOLUTION （ 影响因子：3.342； 五年影响因子：3.188 ）

ISSN： 1567-1348

年卷期： 2019 年 70 卷

页码：

收录情况： SCI

摘要： The centriole in eukaryotes functions as the cell's microtubule-organizing center (MTOC) to nucleate spindle assembly. The evolutionarily conserved protein SAS-6 constitutes the center of the cartwheel assembly that scaffolds centrioles early in their biogenesis. Microsporidia possess the spindle plaque instead of centriole as their MTOC to nucleate spindle assembly. However, little is known about the components of spindle plaques in microsporidia. In our present study, we identified a SAS-6 protein in the microsporidium Nosema bombycis and named it as NSAS-6. The NSAS-6 gene contains a complete ORF of 1104 bp in length that encodes a 367-amino acid polypeptide. NSAS-6 consists of a conserved N-terminal domain and a coiled-coil domain. The high identity of SAS-6 homologous sequences from microsporidia indicates that SAS-6 is a conserved protein in microsporidia. Immunolocalization in sporoplasms, intracellular stages and mature spores showed that NSAS-6 probably localizes to the nucleus of N. bombycis and exists throughout the life cycle of N. bombycis. These results suggest that NSAS-6 is required in cell morphogenesis and division in N. bombycis. The function and structure of NSAS-6 should be the focus for further studies, which is essential to elucidate the role of SAS-6 in spindle plaque assembly.

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