Exogenous Application of Phytohormones Promotes Growth and Regulates Expression of Wood Formation-Related Genes in Populus simonii x P. nigra

文献类型： 外文期刊

第一作者： Yuan, Hongmei

作者： Yuan, Hongmei;Zhao, Lijuan;Zhang, Liguo;Yuan, Hongmei;Yu, Ying;Zhang, Liguo;Song, Xixia;Huang, Wengong;Cheng, Lili;Chen, Jing;Guan, Fengzhi;Wu, Guangwen;Yuan, Hongmei;Li, Huiyu;Zhao, Lijuan;Guo, Wendong;Tao, Lei

作者机构：

关键词： Populus simonii x P; nigra; phytohormone; wood formation related gene; expression profiles; qPCR

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2019 年 20 卷 3 期

页码：

收录情况： SCI

摘要： Although phytohormones are known to be important signal molecules involved in wood formation, their roles are still largely unclear. Here, Populus simonii x P. nigra seedlings were treated with different concentrations of exogenous phytohormones, indole-3-acetic acid (IAA), gibberellin (GA(3)), and brassinosteroid (BR), and the effects of phytohormones on growth were investigated. Next, 27 genes with known roles in wood formation were selected for qPCR analysis to determine tissue-specificity and timing of responses to phytohormone treatments. Compared to the control, most IAA, GA(3), and BR concentrations significantly increased seedling height. Meanwhile, IAA induced significant seedling stem diameter and cellulose content increases that peaked at 3 and 30 mgL(-1), respectively. Significant increase in cellulose content was also observed in seedlings treated with 100 mgL(-1) GA(3). Neither stem diameter nor cellulose content of seedlings were affected by BR treatment significantly, although slight effects were observed. Anatomical measurements demonstrated improved xylem, but not phloem, development in IAA- and BR-treated seedlings. Most gene expression patterns induced by IAA, GA(3), and BR differed among tissues. Many IAA response genes were also regulated by GA(3), while BR-induced transcription was weaker and slower in Populus than for IAA and GA(3). These results reveal the roles played by phytohormones in plant growth and lay the foundation for exploring molecular regulatory mechanisms of wood formation in Populus.

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