文献类型： 外文期刊
第一作者： Zhang, Lingling
作者： Zhang, Lingling;Gao, Dou;Cui, Shangjin;Zhang, Lingling;Gao, Dou;Cui, Shangjin;Yu, Yongle;Liu, Weiquan;Liu, Yebing;Li, Jinxiang
作者机构：
关键词： Porcine parvovirus; Infectious clone; Genetic marker; Rescued virus; Biological properties
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）
ISSN： 0304-8608
年卷期： 2019 年 164 卷 5 期
页码：
收录情况： SCI
摘要： In this study, we describe a novel and rapid method for the construction of a full-length infectious clone (pPPV). The constructed clone contained an engineered EcoRv site that served as a genetic marker and was shown to be infectious when transfected into a monolayer of PK-15 cells. The rescued virus (rPPV) of the infectious clone was found to be indistinguishable from wild-type virus BQ in terms of its biological properties. The generation of this PPV infectious clone provides a potentially powerful tool with which to elucidate the molecular pathogenesis of PPV.
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