Enhancement of immune responses to classical swine fever virus E2 in mice by fusion or mixture with the porcine IL-28B

文献类型： 外文期刊

第一作者： Yuan, Hong

作者： Yuan, Hong;Jiao, Yunjuan;Gao, Jie;Wang, Tao;Li, Kun;Yang, Yuxuan;Zhang, Jing;Bao, Huifang;Wang, Lihao;Sun, Pu;Li, Dong;Li, Pinghua;Cao, Yimei;Zhao, Zhixun;Liu, Zaixin;Lu, Zengjun;Bai, Xingwen;Xia, Yingju;Liu, Yebing;Li, Kun;Cao, Yimei;Lu, Zengjun;Bai, Xingwen

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关键词： Classical swine fever virus; E2 subunit vaccine; IL-28B

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.3； 五年影响因子：5.1 ）

ISSN： 0175-7598

年卷期： 2025 年 109 卷 1 期

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收录情况： SCI

摘要： The E2 subunit vaccine has been considered a promising alternative to an attenuated classical swine fever (CSF) vaccine. However, it fails to induce a good cellular immune response. Given that immunogenic adjuvants can regulate the cellular immunity to achieve a maximum efficacy against antigens, immunostimulatory effects of porcine IL-28B on the CSF virus (CSFV) E2 subunit vaccine were evaluated in the present study. We expressed recombinant proteins E2-IL28B, E2, and IL-28B using CHO-S mammalian cells as an antigen expression platform, and three types of CSFV E2 subunit vaccines based on antigens E2-IL28B, E2 + IL-28B, and E2 were prepared, respectively. We found that both E2-IL28B and E2 + IL-28B antigens exhibited superior immunogenicity with dramatically induced antibody titers and neutralizing antibody levels than the E2 alone. Moreover, E2-IL28B or E2 + IL-28B, instead of E2, boosted cellular immune responses via obviously increasing the percentages of CD3(+)CD4(+) T lymphocytes, promoting the lymphocyte proliferations, and enhancing the release of Th1-type cytokines. All results revealed that the inclusion of IL-28B, whether fused or mixed with E2, significantly elevated E2-induced immune potencies, suggesting that IL-28B could be used as a molecular adjuvant to optimize the design of E2 subunit vaccine for more effective controls of the CSF disease.

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