Simultaneous determination of tocopherols, carotenoids and phytosterols in edible vegetable oil by ultrasound-assisted saponification, LLE and LC-MS/MS

文献类型： 外文期刊

第一作者： Zhang, Liangxiao

作者： Zhang, Liangxiao;Wang, Sujun;Yang, Ruinan;Mao, Jin;Jiang, Jun;Wang, Xiupin;Zhang, Wen;Zhang, Qi;Li, Peiwu;Wang, Sujun;Yang, Ruinan;Mao, Jin;Wang, Xiupin;Zhang, Wen;Zhang, Qi;Li, Peiwu;Zhang, Liangxiao;Li, Peiwu;Zhang, Liangxiao;Jiang, Jun;Zhang, Wen;Zhang, Qi;Li, Peiwu

作者机构：

关键词： Ultrasound-assisted saponification; Liquid-liquid extraction; Central composite design; LC-MS/MS; Bioactive components; Camellia oil

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN： 0308-8146

年卷期： 2019 年 289 卷

页码：

收录情况： SCI

摘要： A method was developed to simultaneously determine eight bioactive compounds in edible oil based on ultrasound-assisted saponification, liquid-liquid extraction and liquid chromatography coupled with tandem mass spectrometry. Central composite design was employed to optimize ultrasonic temperature and time of saponification. Sample treatment was conducted by ultrasound-assisted saponification at temperature of 75 degrees C for 40 min. Limits of detection and limits of quantification ranged from 2.0 to 3.2 and from 6.1 to 10.0 ng/mL, respectively. Linear correlations were obtained (R-2 > 0.99) and the recoveries at three spiked levels were between 81.7% and 112.0%. This method was employed to determine eight compounds in camellia oils and olive oils. As results, the contents of stigmasterol, delta-tocopherol, gamma-tocopherol, beta-carotene and lutein in camellia oils were significantly higher than those in olive oils (p < 0.05). The proposed method can be successfully used to determination of these eight active compounds in camellia oil and other edible oils.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序