Transcriptional Regulation of NAMPT Gene by Glycogen Synthase Kinase 3 beta in Goat Adipocytes

文献类型： 外文期刊

第一作者： Wang, Linjie

作者： Wang, Linjie;Li, Xueying;Zhong, Tao;Guo, Jiazhong;Wang, Yan;Li, Li;Zhang, Hongping;Chao, Zhe

作者机构：

关键词： NAMPT; PPAR gamma; goat adipocyte; GSK3 beta; promoter

期刊名称：DNA AND CELL BIOLOGY （ 影响因子：3.311； 五年影响因子：3.557 ）

ISSN： 1044-5498

年卷期： 2019 年 38 卷 3 期

页码：

收录情况： SCI

摘要： Nicotinamide phosphoribosyl transferase (NAMPT) is a cytokine which is secreted by adipose tissue and involved in the process of immunoreactions, inflammation, and lipid metabolism through the endocrine or paracrine. However, the information regarding NAMPT and its transcriptional regulation in goats remains unknown. In this study, the inactivation of glycogen synthase kinase 3 beta (GSK3 beta) decreased the supernatant NAMPT productions in goat adipocytes. The luciferase activities were kept in high levels in pGL3 (-735/+34) and the deletion from -735 bp to -486 bp significantly decreased the luciferase activity (p < 0.01). In addition, a peroxisome proliferator-activated receptor gamma (PPAR gamma) element in this region was responsible for NAMPT promoter activity. The transcription activity of PPAR gamma was activated by SB216763 stimulation and failed to induce luciferase activity of the pGL3 (Mut-PPAR gamma-735/+34). In contrast, the GSK3 beta overexpression repressed luciferase activity of the NAMPT promoter. Besides, chromatin immunoprecipitation assay indicated that PPAR gamma was capable of binding to the NAMPT promoter, and inhibition of GSK3 beta increased the binding capacity of PPAR gamma to the NAMPT promoter. These results confirm the role of GSK3 beta as a negative regulator of NAMPT transcription in goat adipocytes, and GSK3 beta-regulated expression of NAMPT is mediated by PPAR gamma.

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