Composition of Mitochondrial Complex I during the Critical Node of Seed Aging in Oryza sativa

文献类型： 外文期刊

第一作者： Chen, Baoyin

作者： Chen, Baoyin;Zhang, Zesen;Zhou, Yuanchang;Chen, Baoyin;Yin, Guangkun;Zhang, Zesen;Xin, Xia;He, Juanjuan;Chen, Xiaoling;Zhang, Jinmei;Lu, Xinxiong;Chen, Baoyin;Zhang, Zesen;Zhou, Yuanchang;Whelan, James

作者机构：

关键词： rice; seed aging; mitochondria; respiration; complex I

期刊名称：JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：3.549； 五年影响因子：4.164 ）

ISSN： 0176-1617

年卷期： 2019 年 236 卷

页码：

收录情况： SCI

摘要： Previous studies have documented mitochondrial dysfunction during the critical node (CN) of rice (Oryza sativa) seed aging, including a decrease in the capacity of NADH dependent O-2 consumption. This raises the hypothesis that changes in the activity of NADH: ubiquinone oxidoreductase (complex I) may play a role in seed aging. The composition and activity of complex I was investigated at the CN of aged rice seeds. Using BN-PAGE and SWATH-MS 52 complex I subunits were identified, nineteen for the first time to be experimentally detected in rice. The subunits of the matrix arm (N and Q modules) were reduced in abundance at the CN, in accordance with a reduction in the capacity to oxidise NADH, reducing substrate oxidation and increase ROS accumulation. In contrast, subunits in the P module increased in abundance that contains many mitochondrial encoded subunits. It is proposed that the changes in complex I abundance subunits may indicate a premature re-activation of mitochondrial biogenesis, as evidenced by the increase in mitochondrial encoded subunits. This premature activation of mitochondrial biogenesis may under-pin the decreased viability of aged seeds, as mitochondrial biogenesis is a crucial event in germination to drive growth before autotrophic growth of the seedling is established.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序