Improving Ultra-Low Temperature Preservation Technologies of Soybean Pollen for Off-Season and Off-Site Hybridization

文献类型： 外文期刊

第一作者： Jia, Hongchang

作者： Jia, Hongchang;Liang, Xin;Zhang, Lixin;Zhang, Jinmei;Sapey, Enoch;Sun, Yanhui;Sun, Shi;Han, Tianfu;Jia, Hongchang;Liu, Xianyuan;Yan, Hongrui;Lu, Wencheng;Sapey, Enoch

作者机构：

关键词： soybean; pollen; ultra-low temperature preservation; germination rate; off-site hybridization

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：6.627； 五年影响因子：7.255 ）

ISSN： 1664-462X

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： Preserving viable pollen is of great interest to breeders to maintain desirable germplasm for future inbreeding. Ultra-low temperature preservation of pollen is an effective and safe way for long-term storage of plant germplasm resources. In this study, we improved methods for the preservation of soybean pollen at ultra-low temperature. Soybean flowers at the initially-open stage were collected at 6-10 a.m. during the fully-bloom stage of soybean plants and were dehydrated for 10 h and then frozen and stored at -196 or -80 degrees C. In vitro culture experiments showed that the viability of preserved pollen remained as high as about 90%. The off-season (local site Heihe) and off-site (Beijing, after long-distance express delivery from Heihe) hybridization verification was conducted, and no significant difference in true hybrid rate was founded between the preserved pollen and the fresh pollen. The ultra-low temperature preservation technology for soybean pollen could break the spatiotemporal limit of soybean hybridization and facilitate the development of engineered soybean breeding.

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[1]Improving Ultra-Low Temperature Preservation Technologies of Soybean Pollen for Off-Season and Off-Site Hybridization. Jia, Hongchang,Liang, Xin,Zhang, Lixin,Zhang, Jinmei,Sapey, Enoch,Sun, Yanhui,Sun, Shi,Han, Tianfu,Jia, Hongchang,Liu, Xianyuan,Yan, Hongrui,Lu, Wencheng,Sapey, Enoch. 2022

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