Alanine-substituted mutant on Gly(373) and Asn(375) of Cry1Ai-h-loop 2 causes reduction in both toxicity and binding against Helicoverpa armigera

文献类型： 外文期刊

第一作者： Zhou Zi-shan

作者： Zhou Zi-shan;Liang Ge-mei;Song Fu-ping;Zhang Jie

作者机构：

关键词： Bacillus thuringiensis; Cry1Ai; Domain II-loop2; Helicoverpa armigera; binding affinity

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2019 年 18 卷 5 期

页码：

收录情况： SCI

摘要： Cry1Ai-h-loop 2 is a mutant of Cry1Ai constructed by exchanging loop 2 from Cry1Ah protein and shows insecticidal activity against Helicoverpa armigera. The toxicity of Cry1Ai-h-loop 2, in contrast to the very low toxicity of Cry1Ai, is closely associated with the eleven residues in the loop 2 region. To characterize the key sites of loop 2 in Cry1Ai-h-loop 2, alanine-substituted mutants were generated. The toxicity of these mutants against H. armigera indicated that dual-mutant on Gly(373) and Asn(375) caused a significant decrease in toxic activity. ELISA binding and competition binding assays demonstrated that the reduction of toxicity in the mutant of interest was correlated with decreased binding affinity.

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