An Improved Mesocotyl Elongation Assay for the Rapid Identification and Characterization of Strigolactone-Related Rice Mutants

文献类型： 外文期刊

第一作者： Patil, Suyash

作者： Patil, Suyash;Zafar, Syed Adeel;Uzair, Muhammad;Zhao, Jinfeng;Fang, Jingjing;Li, Xueyong

作者机构：

关键词： mesocotyl; strigolactones; mutagenesis; deep sowing; rice

期刊名称：AGRONOMY-BASEL （ 影响因子：3.417； 五年影响因子：3.64 ）

ISSN： 2073-4395

年卷期： 2019 年 9 卷 4 期

页码：

收录情况： SCI

摘要： Strigolactones (SLs) constitute an important class of plant hormones involved in diverse developmental activities in plant growth and host-parasite interaction. Although substantial progress has been made to understand this pathway, the mechanism of action is still elusive especially with its interaction with other phytohormones and downstream targets. Here we have utilized the negative role of strigolactones in rice (Oryza sativa L.) mesocotyl elongation as a morphological marker for the identification and characterization of new developmental mutants. We observed that deep sown seeds develop longer mesocotyl compared with the surface-grown seeds in the dark condition. Based on this observation, we have developed a method to access mesocotyl elongation consisting of the glass vessel and vermiculite as a growth media. Mesocotyl elongation in the modified deep sown system results in a many-fold increase compared to the surface-grown seeds in the dark condition. External application of SLs analog rac-GR24 rescued the elongated mesocotyl phenotype in the mutant defective in SLs synthesis but not the signaling mutant, demonstrating its applicability in the physiological experiments. The modified mesocotyl elongation assay can be used as a rapid method for characterization and identification of suppressors/enhancers and new developmental mutants in the SLs or its associated pathway saving a huge amount of time and space.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序